Umetskaia V N, Pinaev G P
Institute of Cytology, Russian Academy of Sciences, Tikhoretskii 4, St. Petersburg, 194064 Russia.
Biofizika. 2001 Mar-Apr;46(2):197-202.
The mechanism of transformation of ATP-G actin to F actin by the action of Mg2+ ions was studied by measuring UV absorption spectra of solutions of monomeric Ca(2+)-ATP-G actin in the presence of MgCl2 and of Mg(2+)-ATP-G actin. The protein solutions contained no excessive free ATP. The changes in the UV absorption spectra of monomeric ATP-G actin that result from the interaction with Mg2+ are explained by the formation of ADP-G actin from monomeric ATP-G actin due to hydrolysis of ATP which is considered as the initial step of the transformation to F actin.
通过测量在MgCl₂存在下的单体Ca(2 +)-ATP-G肌动蛋白溶液以及Mg(2 +)-ATP-G肌动蛋白溶液的紫外吸收光谱，研究了Mg²⁺离子作用下ATP-G肌动蛋白向F肌动蛋白转化的机制。蛋白质溶液中不存在过量的游离ATP。单体ATP-G肌动蛋白与Mg²⁺相互作用导致的紫外吸收光谱变化，是由于ATP水解使单体ATP-G肌动蛋白形成了ADP-G肌动蛋白，这被认为是向F肌动蛋白转化的起始步骤。
