[从羽扇豆根瘤中分离多种形式的高铁血红蛋白还原酶和豆血红蛋白成分]

[Isolation of multiple forms of metleghemoglobin reductase and leghemoglobin components from lupin nodules].

作者信息

Shleev S V, Rozov F N, Topunov A F

机构信息

Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 117071 Russia.

出版信息

Prikl Biokhim Mikrobiol. 2001 Mar-Apr;37(2):221-6.

PMID:11357430

Abstract

Method for simultaneous obtaining in homogeneous state of two main forms of metleghemoglobin reductase and main leghemoglobin components from lupine nodules was worked out. The method included steps of saturation with ammonium sulphate (40-80%), gel-filtration on Ultrogel AcA 44, isoelectric focusing and repeated isoelectric focusing. As result the forms of metleghemoglobin reductase with molecular weights 62 and 66 kDa were obtained purified 725 and 402 times respectively and obtained in homogeneous state. The total yield of activity was 37%. The form with 62 kDa molecular weight was more active.

摘要

已制定出从羽扇豆根瘤中同时以均一状态获得高铁血红蛋白还原酶的两种主要形式和主要豆血红蛋白成分的方法。该方法包括用硫酸铵（40 - 80%）饱和、在Ultrogel AcA 44上进行凝胶过滤、等电聚焦和重复等电聚焦步骤。结果分别获得了分子量为62 kDa和66 kDa的高铁血红蛋白还原酶形式，纯化倍数分别为725倍和402倍，并以均一状态获得。活性的总产率为37%。分子量为62 kDa的形式活性更高。