Szybiak-Strózycka U, Strózycki P, Sikorski M, Golińska B, Madrzak C, Legocki A B
Institute of Biochemistry University of Agriculture, Poznań, Poland.
Acta Biochim Pol. 1987;34(2):79-85.
Two yellow lupin leghemoglobins, Lb I and Lb II, were purified to homogeneity using the HPLC technique for final separation. Lb I and Lb II were identified by the N-terminal sequences and their reaction with antibodies against electrophoretically pure leghemoglobin. The third Lb species was detected by the combined method of isoelectrofocusing and PAGE of Lb I. It seems that Lb III represents a posttranslational modification of Lb I. Developmental changes in Lb multiple forms were examined using the Western blotting method. The content of leghemoglobin, first detectable approximately 3 weeks after infection, increased up to 6-7 weeks, and then it remained at the same level until 8-9 weeks after the infection. At the early stages of nodule formation Lb I prevailed over Lb II, while later Lb II became the predominant form. This suggests physiological role of particular forms and precise regulation of the expression of Lb genes.
利用高效液相色谱技术进行最终分离，将两种黄色羽扇豆豆血红蛋白Lb I和Lb II纯化至同质。通过N端序列及其与抗电泳纯豆血红蛋白抗体的反应鉴定Lb I和Lb II。通过Lb I的等电聚焦和聚丙烯酰胺凝胶电泳联合方法检测到第三种Lb种类。Lb III似乎代表Lb I的翻译后修饰。使用蛋白质免疫印迹法检测Lb多种形式的发育变化。豆血红蛋白含量在感染后约3周首次可检测到，在6 - 7周时增加，然后在感染后8 - 9周保持在同一水平。在根瘤形成的早期阶段，Lb I比Lb II占优势，而后期Lb II成为主要形式。这表明特定形式的生理作用以及Lb基因表达的精确调控。
