Rodie C, Sternberg R, Raulin F, Vidal-Madjar C
Laboratoire Interuniversitaire des Systèmes Atmospheriques (LISA), UMR 7583 CNRS, Université Paris 7, Créteil, France.
J Chromatogr A. 2001 Apr 27;915(1-2):199-207. doi: 10.1016/s0021-9673(01)00625-2.
Three different methods of derivatization are tested in order to select and optimize one for the in situ analysis of amino acids in Martian samples. The silylation procedure can easily be automated with a high yield and a linear response in a large range of concentrations. The alkylation method is simple and easily automated, but irreproducible data are obtained for the reaction in the GC liner at quite a high temperature (300 degrees C). Moreover by-products of the reaction interfere in the GC chromatograms and mass spectrometry detection is needed for product identification. The chloroformate derivatization has several advantages such as one-step reaction and short time analysis. The main problem of this procedure is the shaking step which difficult to develop in space application.
为了选择并优化一种用于火星样品中氨基酸原位分析的衍生化方法，对三种不同的衍生化方法进行了测试。硅烷化程序易于自动化，产率高，在很大的浓度范围内具有线性响应。烷基化方法简单且易于自动化，但在相当高的温度（300摄氏度）下，在气相色谱衬管中进行反应时会获得不可重复的数据。此外，反应的副产物会干扰气相色谱图，需要质谱检测来鉴定产物。氯甲酸酯衍生化有几个优点，如一步反应和分析时间短。该程序的主要问题是振荡步骤，这在空间应用中难以实现。
