Antony P, Freysz L, Horrocks L A, Farooqui A A
Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.
Neurochem Res. 2001 Jan;26(1):83-8. doi: 10.1023/a:1007636801035.
LA-N-1 neuroblastoma cell cultures contain Ca2+-independent phospholipases A2 hydrolyzing phosphatidylethanolamine and ethanolamine plasmalogens. These enzymes differ from each other in their molecular mass, substrate specificity, and kinetic properties. Subcellular distribution studies have indicated that the activity of these phospholipases is not only localized in the cytosol but also in non-nuclear membranes and in nuclei. The treatment of LA-N-1 neuroblastoma cell cultures with retinoic acid results in a marked stimulation of Ca2+-independent phospholipases A2 hydrolyzing phosphatidylethanolamine and plasmenylethanolamine. The increase of the activities of both enzymes was first observed in nuclei followed by those present in the cytosol. No effect of retinoic acid on either phospholipase activity could be observed in non-nuclear membranes. The stimulation of these enzymes may be involved in the generation and regulation of arachidonic acid and its metabolites during differentiation.
LA-N-1神经母细胞瘤细胞培养物中含有能水解磷脂酰乙醇胺和乙醇胺缩醛磷脂的钙离子非依赖性磷脂酶A2。这些酶在分子量、底物特异性和动力学特性方面彼此不同。亚细胞分布研究表明，这些磷脂酶的活性不仅定位于细胞质溶胶中，还存在于非核膜和细胞核中。用视黄酸处理LA-N-1神经母细胞瘤细胞培养物会导致能水解磷脂酰乙醇胺和缩醛磷脂酰乙醇胺的钙离子非依赖性磷脂酶A2受到显著刺激。两种酶活性的增加首先在细胞核中观察到，随后在细胞质溶胶中的酶活性也增加。在非核膜中未观察到视黄酸对任何一种磷脂酶活性有影响。这些酶的刺激作用可能参与了分化过程中花生四烯酸及其代谢产物的生成和调节。
