Cubano L A, Lewis M L
Nephrology Section, Department of Medicine, Tulane University Medical Center, and Tulane Environmental Astrobiology Center, New Orleans, Louisiana, USA.
J Leukoc Biol. 2001 May;69(5):755-61.
Heat shock protein levels are increased in cells as a result of exposure to stress. To determine whether heat shock protein regulation could be used to evaluate stress in cells during spaceflight, the response of Jurkat cells to spaceflight and simulated space shuttle launch vibration was investigated by evaluating hsp70 and hsp27 gene expression. Gene expression was assessed by reverse transcription-polymerase chain reaction using mRNA extracted from vibrated, nonvibrated, space-flown, and ground control cells. Results indicate that mechanical stresses of vibration and low gravity do not up-regulate the mRNA for hsp70, although the gene encoding hsp27 is up-regulated by spaceflight but not by vibration. In ground controls, the mRNA for hsp70 and hsp27 increased with time in culture. We conclude that hsp70 gene expression is a useful indicator of stress related to culture density but is not an indicator of the stresses of launch vibration or microgravity. Up-regulation of hsp27 gene expression in microgravity is a new finding.
由于暴露于应激状态，细胞中的热休克蛋白水平会升高。为了确定热休克蛋白调节是否可用于评估太空飞行期间细胞中的应激，通过评估hsp70和hsp27基因表达，研究了Jurkat细胞对太空飞行和模拟航天飞机发射振动的反应。使用从振动、未振动、太空飞行和地面对照细胞中提取的mRNA，通过逆转录-聚合酶链反应评估基因表达。结果表明，振动和低重力的机械应力不会上调hsp70的mRNA，尽管编码hsp27的基因在太空飞行中上调，但在振动中未上调。在地面对照中，hsp70和hsp27的mRNA随培养时间增加。我们得出结论，hsp70基因表达是与培养密度相关的应激的有用指标，但不是发射振动或微重力应激的指标。微重力下hsp27基因表达的上调是一个新发现。
