Ferland C, Guilbert M, Davoine F, Flamand N, Chakir J, Laviolette M
Unité de Recherche en Pneumologie, Centre de Recherche, Hôpital Laval, Institut Universitaire de Cardiologie et Pneumologie de l'Université, Laval, Quebec, Canada.
J Leukoc Biol. 2001 May;69(5):772-8.
The effect of eotaxin, a potent eosinophil chemotactic factor, on eosinophil transmigration through a reconstituted basal membrane (Matrigel) was evaluated. Eotaxin induced significant eosinophil transmigration in the presence of 10% fetal bovine serum (FBS) and interleukin-5. Its effect was optimal at 0.01 microM, and it plateaued at 18 h. Eotaxin's effect was greater with eosinophils from asthmatic subjects (61.1 +/- 3.4%) than with eosinophils from normal subjects (38.7 +/- 4.2%) (P < 0.001). Inhibition of metalloproteinases decreased eotaxin-induced transmigration by < or = 10.4%, whereas inhibition of the plasminogen-plasmin system decreased eotaxin's effect by < or = 44.4% (P = 0.0002). Moreover, eotaxin-induced transmigration was largely diminished in medium with low concentrations of serum [0.5% FBS: 6.1 +/- 2.4%; 10% FBS: 40.2 +/- 5.8% (P = 0.0001)] but returned to its initial level with the addition of plasminogen (2 U/mL) to 0.5% FBS (43.1 +/- 6.5%). These data show that eotaxin is an efficient promoter of eosinophil transmigration in vitro, that it is more potent with cells from asthmatics than with normal cells, and that its effect depends predominantly on the activation of the plasminogen-plasmin system.
评估了一种强效嗜酸性粒细胞趋化因子——嗜酸性粒细胞趋化因子（eotaxin）对嗜酸性粒细胞通过重组基底膜（基质胶）迁移的影响。在存在10%胎牛血清（FBS）和白细胞介素-5的情况下，嗜酸性粒细胞趋化因子可诱导显著的嗜酸性粒细胞迁移。其在0.01微摩尔时效果最佳，并在18小时达到平台期。与来自正常受试者的嗜酸性粒细胞（38.7±4.2%）相比，嗜酸性粒细胞趋化因子对来自哮喘受试者的嗜酸性粒细胞的作用更强（61.1±3.4%）（P<0.001）。金属蛋白酶的抑制使嗜酸性粒细胞趋化因子诱导的迁移减少≤10.4%，而纤溶酶原-纤溶系统的抑制使嗜酸性粒细胞趋化因子的作用减少≤44.4%（P = 0.0002）。此外，在低浓度血清的培养基中[0.5% FBS：6.1±2.4%；10% FBS：40.2±5.8%（P = 0.0001）]，嗜酸性粒细胞趋化因子诱导的迁移大大减少，但在向0.5% FBS中添加纤溶酶原（2 U/mL）后恢复到初始水平（43.1±6.5%）。这些数据表明，嗜酸性粒细胞趋化因子是体外嗜酸性粒细胞迁移的有效促进剂，对哮喘患者的细胞比正常细胞更有效，且其作用主要取决于纤溶酶原-纤溶系统的激活。
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