Kobayashi K, Fukuoka S
Research Institute for Food Sciences, Kyoto University, Uji, Japan.
Arch Biochem Biophys. 2001 Apr 1;388(1):113-20. doi: 10.1006/abbi.2000.2265.
Tamm-Horsfall protein (THP), also referred to as uromodulin, is a major glycoprotein produced by kidney cells. The level of THP in urine is an indicator of renal function. THP levels are difficult to quantify accurately, because the protein forms aggregates in the pH and ion concentrations found in urine. Here, we describe conditions by which THP can be solubilized and kept soluble during measurement using Triton X-100, EDTA, and alkaline pH. Establishing conditions for precise quantification of THP by enzyme-linked immunosorbent assay (ELISA) will aid in studying the functions of THP.
Tamm-Horsfall蛋白（THP），也被称为尿调节蛋白，是一种由肾细胞产生的主要糖蛋白。尿液中THP的水平是肾功能的一个指标。THP水平难以准确量化，因为该蛋白在尿液中的pH值和离子浓度下会形成聚集体。在此，我们描述了使用 Triton X-100、EDTA 和碱性pH值在测量过程中使THP溶解并保持可溶的条件。通过酶联免疫吸附测定（ELISA）建立精确量化THP的条件将有助于研究THP的功能。
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