Kim S J, Ishaque M, Kato L
Microbios. 1978;22(89-90):143-53.
Our earlier studies indicated that the enzyme o-diphenoloxidase was absent in Mycobacterium leprae separated from depromatous human tissues. At that time the bacilli were not available from any other source. The existence or absence of this enzyme in M. leprae recovered from infected armadillo tissues were reinvestigated. The intact cells which were metabolically active, failed to oxidize DOPA. Likewise, DOPA and its derivatives were not oxidized by the enzymatically active cell-free preparations from M. leprae. Upon incubation of DOPA for more than 2 h with whole cell suspensions or particulate fractions, there was no development of colour with an absorption maximum of 540 nm as has been reported for an intermediate of DOPA oxidation. However, DOPA and several phenolic compounds were very actively oxidized by mushroom tyrosinase. The results suggested that M. leprae is deficient in o-diphenoloxidase, and this enzyme is not an intrinsic characteristic of this mycobacterium.
我们早期的研究表明，从脱色斑型麻风病人组织中分离出的麻风分枝杆菌中不存在邻二酚氧化酶。当时无法从任何其他来源获得这些杆菌。我们重新研究了从感染犰狳组织中分离出的麻风分枝杆菌中这种酶的存在与否。代谢活跃的完整细胞无法氧化多巴。同样，多巴及其衍生物也不能被麻风分枝杆菌具有酶活性的无细胞制剂氧化。将多巴与全细胞悬液或颗粒部分孵育超过2小时后，并未出现如报道的多巴氧化中间体在540nm处有最大吸收的颜色变化。然而，多巴和几种酚类化合物能被蘑菇酪氨酸酶非常有效地氧化。结果表明，麻风分枝杆菌缺乏邻二酚氧化酶，且这种酶不是该分枝杆菌的固有特征。
