Mineeva L A, Semënova L R, Gusev M V
Mikrobiologiia. 1979 Jul-Aug;48(4):693-8.
Three periods (the lag period and periods of intensive and decelerated spheroplast formation) can be detected in the action of lysozyme on the cells of Anacystis nidulans; this seems to be due to peculiarities in the cell wall structure of the cyanobacterium and heterogeneity of the culture. EDTA at concentrations of 0.175--0.7 mM has effect on the duration of the lag period but increases the rate of spheroplast formation during the subsequent periods. However, the action of EDTA in complex with lysozyme for 3 hours causes lysis of some of the spheroplasts being formed. The concentration of mannitol below 0.4 M decreases the yield of spheroplasts. The level of intact spheroplasts prepared from A. nidulans suggests that at least part of them is capable of reversing into whole cells.
在溶菌酶作用于集胞藻细胞的过程中，可以检测到三个阶段（延迟期以及原生质球快速形成和减速形成阶段）；这似乎是由于蓝细菌细胞壁结构的特殊性以及培养物的异质性所致。浓度为0.175 - 0.7 mM的乙二胺四乙酸（EDTA）对延迟期的持续时间有影响，但会增加后续阶段原生质球的形成速率。然而，EDTA与溶菌酶共同作用3小时会导致一些正在形成的原生质球裂解。低于0.4 M的甘露醇浓度会降低原生质球的产量。从集胞藻制备的完整原生质球水平表明，其中至少一部分能够逆转为完整细胞。
