van Dyck E, de Deken B, Laga M
STD/HIV Research and Intervention Unit, Department of Microbiology, Institute of Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium.
Int J STD AIDS. 2001 May;12(5):299-301. doi: 10.1258/0956462011923110.
The aim of this study was to evaluate the performance of rapid plasma reagin (RPR) testing using expired and adversely stored antigen reagent. The sensitivity of RPR using antigen stored at 36 degrees C was compared at 3-monthly intervals with RPR using fresh antigen on 116 sera reactive by RPR and by Treponema pallidum particle agglutination (TPPA). After multiple phases of freezing and thawing, 8.3% of initial RPR reactive sera seroreverted. After storage at 36 degrees C for one year and 24 weeks after expiration the overall sensitivity of the adversely stored antigen was 93.8% compared with fresh antigen; the sensitivity was 100% for sera with RPR titres > or = 1:4 and 85.4% for sera with RPR titres of 1:1 and 1:2. The high stability of the reagent may increase the feasibility of the RPR test for use in poorly-equipped healthcare centres in developing countries.
本研究的目的是评估使用过期和储存不当的抗原试剂进行快速血浆反应素（RPR）检测的性能。将在36摄氏度储存的抗原用于RPR检测的敏感性，每隔3个月与使用新鲜抗原的RPR检测进行比较，检测对象为116份经RPR和梅毒螺旋体颗粒凝集试验（TPPA）检测呈反应性的血清。经过多个冻融阶段后，最初RPR反应性血清中有8.3%的血清反应转阴。在36摄氏度储存一年以及过期后24周，储存不当的抗原与新鲜抗原相比总体敏感性为93.8%；RPR滴度≥1:4的血清敏感性为100%，RPR滴度为1:1和1:2的血清敏感性为85.4%。该试剂的高稳定性可能会增加RPR检测在发展中国家设备简陋的医疗中心使用的可行性。
