Van Dyck E, Bogaerts J, Piot P
WHO Collaborating Centre on AIDS, Department of Infection and Immunity, Antwerp, Belgium.
Bull World Health Organ. 1994;72(5):741-3.
Reported are the results of a comparative study of two procedures for the rapid plasma reagin (RPR) test for syphilis serology: in one approach a mechanical rotator was used and in the other, hand rotation was employed. Both procedures were performed on 327 sera. The agreement between both was 98.8%. Three sera that exhibited minimal reactivity (titre = 1) in the mechanical-rotation RPR were nonreactive in the hand-rotation RPR. RPR antigen was stored for 3 months at room temperature (mean, 21 degrees C) and 30 degrees C. There was no difference in the reactivity of 62 sera (50 positive, 12 negative) that were tested using either adversely stored antigens or antigen stored at 4 degrees C. In poorly equipped settings at the primary health care level, the hand-rotation RPR is a practical alternative to mechanical rotation. Also, the stability of the antigen under adverse storage conditions is an additional advantage of RPR for use in tropical areas.
报告了梅毒血清学快速血浆反应素（RPR）试验两种方法的比较研究结果：一种方法使用机械旋转器，另一种方法采用手动旋转。两种方法均对327份血清进行了检测。两者之间的一致性为98.8%。在机械旋转RPR中表现出最小反应性（滴度=1）的3份血清在手动旋转RPR中无反应。RPR抗原在室温（平均21摄氏度）和30摄氏度下储存3个月。使用储存条件不利的抗原或在4摄氏度下储存的抗原检测的62份血清（50份阳性，12份阴性）的反应性没有差异。在初级卫生保健水平设备简陋的环境中，手动旋转RPR是机械旋转的一种实用替代方法。此外，抗原在不利储存条件下的稳定性是RPR在热带地区使用的另一个优势。
