前列腺素A1、亚砷酸盐和阿司匹林对蚊虫细胞应激蛋白反应的影响。

Effect of prostaglandin A1, arsenite and aspirin on stress proteins response in mosquito cells.

作者信息

de Meneses M D, Rebello M A

机构信息

Departamento de Virologia, Instituto de Microbiologia Professor Paulo de Goés, Universidade Federal do Rio de Janeiro, Brasil.

出版信息

Z Naturforsch C J Biosci. 2001 Mar-Apr;56(3-4):298-302. doi: 10.1515/znc-2001-3-421.

DOI:10.1515/znc-2001-3-421

PMID:11371024

Abstract

The stress response of eukaryotic cells is characterized by changes in the metabolism of responding cells, most notably by increased synthesis of a group of proteins known as heat shock (HSP) proteins In this paper the effect of prostaglandin A1 (PGA1), arsenite and aspirin in Aedes albopictus cells was investigated. In cells treated with PGA1 (10 microg/ml) we observed the induction of several polypeptides with molecular masses of 87, 80, 70, 57, 29 and 23 kDa. Immunoblot analysis revealed that arsenite induces a marked synthesis of HSP70, and aspirin administered during the hyperthermic treatment caused a small increase of HSP70 synthesized.

摘要

真核细胞的应激反应以反应细胞代谢的变化为特征，最显著的是一组称为热休克（HSP）蛋白的蛋白质合成增加。本文研究了前列腺素A1（PGA1）、亚砷酸盐和阿司匹林对白纹伊蚊细胞的影响。在用PGA1（10微克/毫升）处理的细胞中，我们观察到诱导出了几种分子量分别为87、80、70、57、29和23千道尔顿的多肽。免疫印迹分析显示，亚砷酸盐诱导HSP70的显著合成，而在热疗期间给予阿司匹林会使合成的HSP70略有增加。