一例良性局限性胸膜间皮瘤中透明质酸的生化特性分析

Biochemical characterization of hyaluronic acid from a case of benign, localized, pleural mesothelioma.

作者信息

Motomiya M, Endo M, Arai H, Yokosawa A, Sato H, Konno K

出版信息

Am Rev Respir Dis. 1975 Jun;111(6):775-80. doi: 10.1164/arrd.1975.111.6.775.

DOI:10.1164/arrd.1975.111.6.775

Abstract

The tissue of a benign, localized, pleural mesothelioma was digested with protease, and the crude polysaccharide was fractionated by a column of Dowex-1 (Cl-form). The eluate from the column was electrophoresed and incubated with various mucopolysaccharide lysases. Based on the results of column chromatography, electrophoresis, and enzymatic digestion, it was found that hyaluronic acid was the major constituent of the glycosaminoglycans in pleural mesothelioma. The hyaluronic acid from pleural mesothelioma seemed to be identical in structure with that from human umbilical cord; however, the hyaluronic acid from mesothelioma was eluted before that from human umbilical cord when fractionated by the column of Sepharose 4B, suggesting a differnence in molecular size between the two. Also, evidence was obtained for the presence of dermatan sulfate and heparan sulfate.

摘要

将良性局限性胸膜间皮瘤组织用蛋白酶消化，粗多糖用Dowex - 1（Cl型）柱进行分级分离。将柱洗脱液进行电泳，并与各种粘多糖裂解酶一起孵育。基于柱色谱、电泳和酶消化的结果，发现透明质酸是胸膜间皮瘤中糖胺聚糖的主要成分。胸膜间皮瘤中的透明质酸在结构上似乎与人脐带中的透明质酸相同；然而，当用Sepharose 4B柱分级分离时，间皮瘤中的透明质酸比人脐带中的透明质酸先被洗脱，这表明两者分子大小存在差异。此外，还获得了存在硫酸皮肤素和硫酸乙酰肝素的证据。