Yoshikawa T, Naruse S, Kitagawa M, Ishiguro H, Nagahama M, Yasuda E, Semba R, Tanaka M, Nomura K, Hayakawa T
Department of Internal Medicine II, Nagoya University School of Medicine, Nagoya, Japan.
J Histochem Cytochem. 2001 Jun;49(6):777-82. doi: 10.1177/002215540104900611.
It has been known that group II phospholipase A2 (PLA2) mRNA and protein are present in the homogenates of the spleen, lung, liver, and kidney in normal rats, but the cellular origin of this enzyme has not been yet identified. At present, five subtypes of group II PLA2 have been identified in mammals. Antibodies or mRNA probes previously used for detecting group II PLA2 need to be evaluated to identify the subtypes of group II PLA2. In this study we tried to identify group IIA PLA2-producing cells in normal rat tissues by in situ hybridization (ISH) using an almost full-length RNA probe for rat group IIA enzyme. Group IIA PLA2 mRNA was detected in megakaryocytes in the spleen and Paneth cells in the intestine by ISH. These cells were also immunopositive for an antibody raised against group IIA PLA(2) isolated from rat platelets. Group IIA PLA2 mRNA-positive cells were not detected in lung, liver, kidney, and pancreas. Under normal conditions, group IIA PLA2-producing cells are splenic megakaryocytes and intestinal Paneth cells in rats.
已知在正常大鼠中，脾脏、肺、肝脏和肾脏的匀浆中存在II型磷脂酶A2（PLA2）的mRNA和蛋白质，但该酶的细胞来源尚未确定。目前，在哺乳动物中已鉴定出五种II型PLA2亚型。需要对先前用于检测II型PLA2的抗体或mRNA探针进行评估，以鉴定II型PLA2的亚型。在本研究中，我们试图通过原位杂交（ISH），使用针对大鼠IIA型酶的几乎全长RNA探针，来鉴定正常大鼠组织中产生IIA型PLA2的细胞。通过ISH在脾脏的巨核细胞和肠道的潘氏细胞中检测到了IIA型PLA2 mRNA。这些细胞对针对从大鼠血小板中分离出的IIA型PLA(2)产生的抗体也呈免疫阳性。在肺、肝脏、肾脏和胰腺中未检测到IIA型PLA2 mRNA阳性细胞。在正常条件下，大鼠中产生IIA型PLA2的细胞是脾脏巨核细胞和肠道潘氏细胞。
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