鸡肠磷脂酶 A2 的抗菌特性。
Antibacterial properties of chicken intestinal phospholipase A2.
机构信息
Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS Route de Soukra, 3038 Sfax, University of Sfax, Tunisia.
出版信息
Lipids Health Dis. 2011 Jan 12;10:4. doi: 10.1186/1476-511X-10-4.
BACKGROUND
The presence of chicken group-IIA PLA2 (ChPLA2-IIA) in the intestinal secretion suggests that this enzyme plays an important role in systemic bactericidal defence. We have analyzed the bactericidal activity of purified ChPLA2-IIA, on several gram-positive and gram-negative bacteria by using the diffusion well and dilution methods.
RESULTS
ChPLA2-IIA displays potent bactericidal activity against gram-positive bacteria but lacks bactericidal activity against gram negative ones. We have also demonstrated a synergic action of ChPLA2-IIA with lysozyme when added to the bacteria culture prior to ChPLA2-IIA. The bactericidal efficiency of ChPLA2-IIA was shown to be dependent upon the presence of calcium ions and then a correlation could be made to its hydrolytic activity of membrane phospholipids. Interestingly ChPLA2-IIA displays a higher dependence to Ca²+ ions than to Mg²+ ions.
CONCLUSION
We conclude that the main physiological role of ChPLA2-IIA could be the defence of the intestine against bacterial invasions.
背景
肠道分泌物中存在鸡 IIA 组 PLA2（ChPLA2-IIA）表明这种酶在全身杀菌防御中发挥重要作用。我们使用扩散孔和稀释方法分析了纯化的 ChPLA2-IIA 对几种革兰氏阳性和革兰氏阴性细菌的杀菌活性。
结果
ChPLA2-IIA 对革兰氏阳性菌显示出强大的杀菌活性，但对革兰氏阴性菌没有杀菌活性。我们还证明了 ChPLA2-IIA 与溶菌酶协同作用，当在添加 ChPLA2-IIA 之前加入细菌培养物中时。ChPLA2-IIA 的杀菌效率取决于钙离子的存在，然后可以将其与膜磷脂的水解活性相关联。有趣的是，ChPLA2-IIA 对 Ca²+离子的依赖性高于对 Mg²+离子的依赖性。
结论
我们得出结论，ChPLA2-IIA 的主要生理作用可能是防御肠道免受细菌入侵。
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