Rang C, Vachon V, Coux F, Carret C, Moar W J, Brousseau R, Schwartz J L, Laprade R, Frutos R
CIRAD, TA 40/PS1, Boulevard de la Lironde, 34398 Montpellier Cedex 5, France.
Curr Microbiol. 2001 Jul;43(1):1-6. doi: 10.1007/s002840010250.
Influence of domain I exchange on the stability and production of Bacillus thuringiensis Cry1 protoxins as well as on the shape of inclusion and toxicity to Spodoptera exigua and Plutella xylostella larvae was investigated. Chimeric genes were prepared by exchanging the regions coding for domain I between Cry1Aa, Cry1Ab, Cry1Ac, Cry1C, and Cry1E. The AcCC chimera accumulated into bipyramidal inclusion bodies, whereas CEE produced round-shaped inclusion bodies, and ECC and AaEE protoxins produced small granules. AbEE and EAaAa did not produce any inclusion body and were visualized by immunodetection only. AcCC, CEE, ECC, and AaEE were stable to trypsin, whereas AbEE and EAaAa were not. Bioassays showed that the chimeras were not toxic in vivo. However, S. exigua larvae fed with the activated AcCC toxin displayed a lower growth rate.
研究了结构域I交换对苏云金芽孢杆菌Cry1原毒素稳定性和产量的影响，以及对包涵体形状和对甜菜夜蛾和小菜蛾幼虫毒性的影响。通过交换Cry1Aa、Cry1Ab、Cry1Ac、Cry1C和Cry1E之间编码结构域I的区域制备嵌合基因。AcCC嵌合体积累成双金字塔形包涵体，而CEE产生圆形包涵体，ECC和AaEE原毒素产生小颗粒。AbEE和EAaAa不产生任何包涵体，仅通过免疫检测可视化。AcCC、CEE、ECC和AaEE对胰蛋白酶稳定，而AbEE和EAaAa不稳定。生物测定表明，嵌合体在体内无毒。然而，用活化的AcCC毒素喂养的甜菜夜蛾幼虫生长速率较低。
