Akagi K, Yamamura K
Saitama Cancer Center Research Institute, Ina, Kitaadachigun, Saitama 362-0806, Japan.
Jpn J Cancer Res. 2001 May;92(5):499-505. doi: 10.1111/j.1349-7006.2001.tb01122.x.
We previously showed that B and T cell lymphoma development in Em (immunoglobulin heavy chain enhancer)-myc transgenic mice is dependent on the mouse strain. To determine whether any non-random chromosomal abnormality that was present was caused by variations in the lymphoma cell type or by a different genetic background, we crossed C3H transgenic mice with other inbred strains of mice, C57BL / 6 or BALB / c. Cytogenetic analysis showed a high frequency of non-random chromosomal aberrations, namely, duplication or amplification of part of chromosome 5 containing the transgene and trisomy of chromosome 1, 6, or 12 in the genetic background of C3H x C57BL / 6 mouse and C3H x BALB / c mouse, respectively, regardless of cell type of lymphoma. These results suggest that non-random chromosomal abnormalities in lymphoma cells are dependent on the genetic background of mouse, not on the tumor cell type in Em-myc transgenic mouse.
我们之前表明，Em（免疫球蛋白重链增强子）-myc转基因小鼠中B细胞和T细胞淋巴瘤的发生取决于小鼠品系。为了确定存在的任何非随机染色体异常是由淋巴瘤细胞类型的差异还是不同的遗传背景引起的，我们将C3H转基因小鼠与其他近交系小鼠C57BL / 6或BALB / c进行杂交。细胞遗传学分析显示，在C3H×C57BL / 6小鼠和C3H×BALB / c小鼠的遗传背景中，分别存在高频率的非随机染色体畸变，即包含转基因的5号染色体部分的重复或扩增以及1号、6号或12号染色体的三体性，而与淋巴瘤的细胞类型无关。这些结果表明，淋巴瘤细胞中的非随机染色体异常取决于小鼠的遗传背景，而不是Em-myc转基因小鼠中的肿瘤细胞类型。
