Al-Momani I F
Chemistry Department, Yarmouk University, Irbid, Jordan.
J Pharm Biomed Anal. 2001 Jul;25(5-6):751-7. doi: 10.1016/s0731-7085(01)00368-5.
A sensitive, accurate and rapid flow injection analysis (FIA) method for the determination of cefotaxime, cefuroxime, ceftriaxone, cefaclor, cefixime, ceftizoxime, and cephalexin is proposed. Aliquots of each cephalosporin were hydrolyzed for 15 min with 0.1 M NaOH at 80 degrees C and then oxidized with Fe3+ in sulfuric acid medium to produce Fe2+. The produced Fe2+ is then complexed by o-phenanthroline (o-phen) in citrate buffer at pH 4.2 to form the red complex, Fe(o-phen)3(2+), which exhibits an absorption maximum at 510 nm. Variables such as acidity, reagent concentrations, flow rate of reagents and other FI parameters were optimized to produce the most sensitive and reproducible results. The method was successfully applied to the analysis of pharmaceutical preparations. The results have been compared with those obtained using the official methods. Excellent agreement between the results of the proposed method and the official methods was obtained.
本文提出了一种灵敏、准确且快速的流动注射分析(FIA)方法,用于测定头孢噻肟、头孢呋辛、头孢曲松、头孢克洛、头孢克肟、头孢唑肟和头孢氨苄。将每种头孢菌素的等分试样在80℃下用0.1 M氢氧化钠水解15分钟,然后在硫酸介质中用Fe3+氧化以生成Fe2+。生成的Fe2+随后在pH 4.2的柠檬酸盐缓冲液中与邻菲啰啉(o-phen)络合形成红色络合物Fe(o-phen)3(2+),该络合物在510 nm处有最大吸收。对酸度、试剂浓度、试剂流速和其他流动注射参数等变量进行了优化,以获得最灵敏且可重复的结果。该方法成功应用于药物制剂的分析。已将结果与使用官方方法获得的结果进行了比较。所提出的方法与官方方法的结果之间取得了极好的一致性。