Shrivastava Sanjay Mohan, Singh Rajkumar, Tariq Abu, Siddiqui Masoom Raza, Yadav Jitendar, Negi P S, Chaudhary Manu
Venus Medicine Research Centre, Hill Top Industrial Estate, EPIP Phase I, Jharmajri (Extn.) Bhatoli Kalan, Baddi, Solan (H. P.), India.
Int J Biomed Sci. 2009 Mar;5(1):37-43.
An isocratic liquid chromatographic method with UV detection at 220 nm is described for simultaneous determination of ceftriaxone sodium and sulbactam sodium in Sulbactomax. Chromatographic separation of two drugs was achieved on a Hypersil ODS C-18 column using a mobile phase consisting of a binary mixture of acetonitrile and tetrabutyl ammonium hydroxide adjusted to pH7.0 with orthophosphoric acid in ratio 70:30. The developed Liquid Chromatographic method offers symmetric peak shape, good resolution and reasonable retention time for both drugs. Linearity, accuracy and precision were found to be acceptable over the concentration range of 125-750 ppm for ceftriaxone sodium and 62.5-375 ppm for sulbactam sodium. The LC method can be used for the quality control of formulated products containing ceftriaxone and sulbactam.
本文描述了一种采用等度液相色谱法，在220nm波长处进行紫外检测，用于同时测定舒普深中头孢曲松钠和舒巴坦钠含量的方法。两种药物在Hypersil ODS C-18柱上实现色谱分离，流动相由乙腈和氢氧化四丁铵的二元混合物组成，用正磷酸调节pH值至7.0，比例为70:30。所建立的液相色谱方法为两种药物提供了对称的峰形、良好的分离度和合理的保留时间。头孢曲松钠在125 - 750 ppm浓度范围内、舒巴坦钠在62.5 - 375 ppm浓度范围内，线性、准确度和精密度均符合要求。该液相色谱法可用于含头孢曲松和舒巴坦制剂产品的质量控制。
