Integrated bioprocess for enhanced production of natural flavors and fragrances by Ceratocystis moniliformis.

An integrated bioprocess (IBP) for production and recovery of de novo synthesized aroma compounds was carried out by interlinking a pervaporation membrane module with a producing bioreactor. The main aroma products of the fungus Ceratocystis moniliformis were ethyl acetate, propyl acetate, isobutyl acetate, isoamyl acetate, citronellol and geraniol. In situ product removal (ISPR) using pervaporation leads to decreased product concentrations in the bioreactor and increased microbial growth rates. As a result, by circumventing inhibiting product concentrations and thus intensifying aroma production, total yield of aroma compounds produced is higher in an IBP compared with batch cultivation. In addition, permeates obtained from pervaporation consist of highly enriched mixtures of produced flavors and fragrances.