Pinheiro Carmen, Rodrigues Carla M, Schäfer Thomas, Crespo João G
Chemistry Department, CQFB, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
Biotechnol Bioeng. 2002 Mar 20;77(6):632-40. doi: 10.1002/bit.10141.
This work discusses the feasibility of using the electronic nose for the on-line and real-time monitoring of the production of a complex aroma profile during a bioconversion process. As a case study, the formation of the muscatel aroma during the wine-must fermentation was selected. During wine-must fermentation, aroma compounds responsible for the organoleptic character are produced in the ppm range, while simultaneously one of the main metabolic products, ethanol, is produced in much higher quantities (up to 10% wt). Because the sensors of the electronic nose array are cross-selective to different volatile compounds, it was investigated in detail how far the electronic nose was able to evaluate the aroma profile along the fermentation. This article discusses and evaluates subsequently the integration of a membrane separation process-organophilic pervaporation-for selectively enriching aroma compounds relative to ethanol, to improve sample discrimination.
本研究探讨了使用电子鼻对生物转化过程中复杂香气特征生成进行在线实时监测的可行性。以葡萄酒发酵过程中麝香葡萄香气的形成为案例研究。在葡萄汁发酵过程中,赋予感官特征的香气化合物的生成量处于ppm级别,而与此同时,主要代谢产物之一乙醇的生成量要高得多(高达10%重量)。由于电子鼻阵列的传感器对不同挥发性化合物具有交叉选择性,因此详细研究了电子鼻在多大程度上能够评估整个发酵过程中的香气特征。本文随后讨论并评估了膜分离过程——亲有机渗透蒸发——相对于乙醇选择性富集香气化合物以改善样品区分的整合情况。