Kloppel T M, Post G
J Nutr. 1975 Jul;105(7):861-6. doi: 10.1093/jn/105.7.861.
Tryptophan-deficient and nondeficient synthetic amino acid test diets were prepared using D, L-amino acids. The diets were fed to three groups of rainbow trout (Salmo gairdneri) for 8 weeks. Experimental control fish fed well and grew from 1.3 to 2.5 g. Deficient fish fed poorly and did not gain weight. Scoliosis was observed in the deficient fish after 1 week of feeding. Daily transitory scoliosis was noted in some fish. Histological studies of trypthophan-deficient scoliotic fish revealed hyperemia, disorganization of myomere septa, and protrusions of the fibrous matrix sheath, which invests the notochord. Abnormal deposition of calcium was noted in the kidney and the bony plates surrounding the notochord and sheath. Fish with mechanically induced scoliosis had disorganization of myomere septa, but did not have protrusions of fibrous matrix sheath, nor did they have abnormal calcium deposition in bone or kidney. Scoliotic fish returned to normal within 1 week upon replacement of tryptophan in the ration.
使用D，L-氨基酸制备了色氨酸缺乏和非缺乏的合成氨基酸试验日粮。将这些日粮投喂给三组虹鳟（Salmo gairdneri）8周。实验对照组的鱼生长良好，体重从1.3克增加到2.5克。缺乏色氨酸的鱼摄食不佳，体重未增加。在投喂1周后，在缺乏色氨酸的鱼中观察到脊柱侧凸。在一些鱼中还注意到每日短暂性脊柱侧凸。对缺乏色氨酸的脊柱侧凸鱼进行组织学研究发现，存在充血、肌节隔膜紊乱以及围绕脊索的纤维基质鞘突出。在肾脏以及围绕脊索和鞘的骨板中发现了钙的异常沉积。机械诱导脊柱侧凸的鱼存在肌节隔膜紊乱，但没有纤维基质鞘突出，在骨骼或肾脏中也没有钙的异常沉积。当在日粮中补充色氨酸后，脊柱侧凸的鱼在1周内恢复正常。
