Stitou S, Jiménez R, Díaz De La Guardia R, Burgos M
Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain.
Heredity (Edinb). 2001 Feb;86(Pt 2):128-33. doi: 10.1046/j.1365-2540.2001.00771.x.
We analysed the karyotype of the rodent species Apodemus sylvaticus by G- and C-banding, Ag-NOR-staining and in situ hybridization, with special attention to the sex chromosomes. NOR-bearing chromosome pairs were identified with simultaneous staining of G bands and NORs. In situ hybridization with an rDNA probe revealed the presence of silent ribosomal cistrons in both sex chromosomes. Studies of meiosis demonstrated that these inactive ribosomal cistrons are located in the pairing segment, which occupies the proximal portion of the telocentric sex chromosomes, and may thus be involved in postreduction caused by an obligatory chiasma in this position.
我们通过G带和C带、银染核仁组织区（Ag-NOR）染色及原位杂交分析了啮齿动物林姬鼠的核型，特别关注了性染色体。通过G带和核仁组织区的同时染色鉴定出携带核仁组织区的染色体对。用rDNA探针进行原位杂交揭示了两条性染色体中均存在沉默核糖体顺反子。减数分裂研究表明，这些无活性的核糖体顺反子位于配对区段，该配对区段占据了端着丝粒性染色体的近端部分，因此可能参与了由该位置的一个 obligatory 交叉所导致的后减数分裂。
