Emery A M, Wilson I J, Craig S, Boyle P R, Noble L R
University of Aberdeen, Department of Zoology, Tillydrone Avenue, Aberdeen, AB24 2TZ, UK.
Mol Ecol. 2001 May;10(5):1265-78. doi: 10.1046/j.1365-294x.2001.01258.x.
We present a novel approach to investigating sibling relationships and reconstructing parental genotypes from a progeny array. The Bayesian method we have employed is flexible and may be applicable to a variety of situations in addition to the one presented here. While mutation rates and breeding population allele frequencies can be taken into account, the model requires relatively few loci and makes few assumptions. Paternity of 270 veined squid (Loligo forbesi) hatchlings from three egg strings collected from one location was assigned using five microsatellite loci. Paternal and maternal genotypes reconstructed for each of the three strings were identical, strongly indicating that a single female produced the strings that were fertilized by the same four males. The proportion of eggs fertilized was not equal between males in all three strings, with male 1 siring most offspring (up to 68% in string 1), through to male 4 siring the least (as low as 2.4% in string 1). Although temperature had a profound effect on incubation time, paternity did not affect this trait at 12 degrees C or 8 degrees C.
我们提出了一种新方法，用于研究同胞关系并从子代阵列中重建亲本基因型。我们采用的贝叶斯方法具有灵活性，除了本文介绍的情况外，还可能适用于各种情形。虽然可以考虑突变率和繁殖群体等位基因频率，但该模型所需的基因座相对较少，且假设也较少。使用五个微卫星基因座对从一个地点采集的三个卵串中的270只脉管鱿鱼（Loligo forbesi）幼体进行了父系鉴定。为三个卵串中的每一个重建的父本和母本基因型是相同的，这有力地表明，单个雌性产出了由相同四只雄性受精的卵串。在所有三个卵串中，雄性之间受精的卵的比例并不相等，雄性1使大多数后代受精（在卵串1中高达68%），而雄性4使受精的后代最少（在卵串1中低至2.4%）。虽然温度对孵化时间有深远影响，但在12摄氏度或8摄氏度时，父系情况并不影响这一性状。
