Preuss M, Leonhard K, Hell K, Stuart R A, Neupert W, Herrmann J M
Insitute for Phyisiological Chemistry, Goethestrasse 33, 80336 Münich, Germany.
J Cell Biol. 2001 May 28;153(5):1085-96. doi: 10.1083/jcb.153.5.1085.
The biogenesis of mitochondria requires the integration of many proteins into the inner membrane from the matrix side. The inner membrane protein Oxa1 plays an important role in this process. We identified Mba1 as a second mitochondrial component that is required for efficient protein insertion. Like Oxa1, Mba1 specifically interacts both with mitochondrial translation products and with conservatively sorted, nuclear-encoded proteins during their integration into the inner membrane. Oxa1 and Mba1 overlap in function and substrate specificity, but both can act independently of each other. We conclude that Mba1 is part of the mitochondrial protein export machinery and represents the first component of a novel Oxa1-independent insertion pathway into the mitochondrial inner membrane.
线粒体的生物合成需要许多蛋白质从基质侧整合到内膜中。内膜蛋白Oxa1在这一过程中发挥着重要作用。我们鉴定出Mba1是高效蛋白质插入所必需的第二个线粒体成分。与Oxa1一样，Mba1在将线粒体翻译产物和保守分选的核编码蛋白质整合到内膜的过程中，都能与它们特异性相互作用。Oxa1和Mba1在功能和底物特异性上存在重叠，但两者均可独立发挥作用。我们得出结论，Mba1是线粒体蛋白质输出机制的一部分，并且代表了一种不依赖于Oxa1的插入线粒体内膜新途径的首个成分。
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