Galebskaia L V, Solovtsova I L, Riumina E V
Saint-Petersburg State Pavlov Medical University.
Vopr Med Khim. 2001 Jan-Feb;47(1):91-7.
The influence of heparin therapeutic concentrations (0.25-10 U/ml) on the kinetic indices of human serum complement activity has been studied. An acceleration of complement-dependent lysis of rabbit erythrocytes via alternative complement pathway was found at low (0.25-0.5 U/ml) heparin concentrations and complement inhibition at higher (5-10 U/ml) concentration. The alternative pathway inhibition was revealed mainly by lag-period elongation of the hemolysis. Heparin in the concentrations more then 1 U/ml caused dose-dependent lowering of sheep erythrocytes hemolysis rate accompanied with reciprocal elevation of complement hemolytical capacity, which shows more "economical" consumption of complement components at heparin presence. The identical effect was observed in sera, deficient in factor D of alternative pathway of complement activation. This can be an evidence of the prevailing heparin inhibition of the classical pathway.
研究了肝素治疗浓度（0.25 - 10 U/ml）对人血清补体活性动力学指标的影响。在低肝素浓度（0.25 - 0.5 U/ml）下，发现通过替代补体途径加速了兔红细胞的补体依赖性溶解，而在较高浓度（5 - 10 U/ml）时则出现补体抑制。替代途径的抑制主要表现为溶血延迟期延长。浓度超过1 U/ml的肝素导致绵羊红细胞溶血率呈剂量依赖性降低，同时补体溶血能力相应升高，这表明在有肝素存在时补体成分的消耗更“经济”。在补体激活替代途径中缺乏D因子的血清中也观察到了相同的效果。这可能是肝素对经典途径具有主要抑制作用的证据。
