Melton T, Nelson K
Mitotyping Technologies, LLC, 1981 Pine Hall Drive, State College, PA 16801, USA.
Croat Med J. 2001 Jun;42(3):298-303.
To describe mitochondrial DNA (mtDNA) forensic casework experience in a commercial laboratory in the United States.
Frequency statistics were kept for two years on all aspects of mtDNA forensic cases, including types of clientele, types of samples, levels of sample success and failure, site heteroplasmy, length heteroplasmy, contamination, rates of failures to exclude, and match statistics using a mtDNA sequence database.
Low sample failure rate was observed, especially since an "ancient DNA" approach was used for samples with degraded DNA. Levels of contamination were low, and the observed site and length heteroplasmy did not confound the interpretation of results. The data collected from mtDNA haplotypes developed in casework showed extremely high diversity of haplotypes consistent with other formally developed databases.
MtDNA forensic analysis in the private sector was successfully applied to many different types of samples overall, with minimal rates of complication due to sample handling challenges (degraded DNA, minimal samples, contamination) and sequence-specific phenomena (site and length heteroplasmy).
描述美国一家商业实验室中线粒体DNA（mtDNA）法医案件工作的经验。
对mtDNA法医案件的各个方面进行了为期两年的频率统计，包括客户类型、样本类型、样本成功与失败水平、位点异质性、长度异质性、污染、排除失败率以及使用mtDNA序列数据库的匹配统计。
观察到样本失败率较低，特别是自从对降解DNA样本采用“古代DNA”方法以来。污染水平较低，观察到的位点和长度异质性并未混淆结果的解释。从案件工作中开发的mtDNA单倍型收集的数据显示，单倍型具有极高的多样性，与其他正式开发的数据库一致。
总体而言，私营部门的mtDNA法医分析成功应用于许多不同类型的样本，由于样本处理挑战（降解DNA、微量样本、污染）和序列特异性现象（位点和长度异质性）导致的并发症发生率极低。
