Kucharska U, Kuczyński W, Pietrewicz P, Wasilewski T
Oddziału Rehabilitacji Leczniczej Wojewódzkiego Szpitala Zespolonego w Białymstok.
Ginekol Pol. 2001 Jan;72(1):31-5.
Semen was obtained by transrectal electrostimulation from male patient after retroperitoneal lymph node dissection who had not ejaculated since time of operation. Vibratory stimulation was unsuccessful. electroejaculation was done under general anaesthesia. A silicone catheter was used to tamponade the bladder neck and antegrade ejaculations were collected. The semen underwent seminological assessment and then was diluted 1:1 in egg yolk substrate containing 15% glycerol, equilibrated, divided into straws and frozen in nitrogen. The straws were then placed for storage in liquid nitrogen until the insemination.
通过经直肠电刺激从一名腹膜后淋巴结清扫术后未射精的男性患者获取精液。振动刺激未成功。在全身麻醉下进行电射精。使用硅胶导管压迫膀胱颈并收集顺行射精的精液。对精液进行精液学评估，然后在含有15%甘油的蛋黄基质中按1:1稀释，平衡后，分装到细管中并在液氮中冷冻。然后将细管置于液氮中储存直至授精。
