Carpentier S M, Shen M, Chen L, Cunanan C M, Martinet B, Carpentier A
Laboratoire d'Etude des Greffes et Prothèses Cardiaques, Hĵpital Européen Georges Pompidou, Paris, France.
Ann Thorac Surg. 2001 May;71(5 Suppl):S410-2. doi: 10.1016/s0003-4975(01)02494-8.
Preliminary studies showed that heat treatment of glutaraldehyde preserved valvular bioprostheses mitigates calcification. This study was carried out to define the physicochemical characteristics of the heat-treated tissues to elucidate the mechanism involved in the mitigation.
Glut bovine pericardium or porcine valve samples were treated at 50 degrees C in a 0.625% glutaraldehyde solution for 2 months. Some samples underwent assay for shrinkage temperature, moisture content, ninhydrin test, and acid hydrolysis, and other samples were incubated in human serum for 3 days and then analyzed by electrophoresis to study protein adsorption.
Heat treatment mitigated calcification without adversely affecting shrinkage temperature (84.81 degrees C versus 83.95 degrees C) and moisture content (78.68% versus 78.71%). A significant reduction in free amino groups (0.15 versus 0.37 mol NH2/mol collagen) and a significant increase in resistance to acid hydrolysis were observed. Total protein content was similar, but significant differences were found for four proteins adsorbed in the tissues (167, 45, 11.6, and 10 kDa).
The anticalcification effect of heat treatment may be attributed to structural changes, lipid extraction, increased resistance, and modifications of the type and concentration of the proteins adsorbed in the tissue.
初步研究表明，对戊二醛保存的心脏瓣膜生物假体进行热处理可减轻钙化。本研究旨在确定热处理组织的物理化学特性，以阐明减轻钙化所涉及的机制。
将戊二醛处理的牛心包或猪瓣膜样本在0.625%的戊二醛溶液中于50摄氏度下处理2个月。一些样本进行了收缩温度、水分含量、茚三酮试验和酸水解测定，其他样本在人血清中孵育3天，然后通过电泳分析以研究蛋白质吸附情况。
热处理减轻了钙化，且未对收缩温度（84.81摄氏度对83.95摄氏度）和水分含量（78.68%对78.71%）产生不利影响。观察到游离氨基显著减少（0.15对0.37摩尔NH2/摩尔胶原蛋白），对酸水解的抗性显著增加。总蛋白含量相似，但在组织中吸附的四种蛋白质（167、45、11.6和10 kDa）存在显著差异。
热处理的抗钙化作用可能归因于结构变化、脂质提取、抗性增加以及组织中吸附蛋白质的类型和浓度的改变。
