在甲状腺激素受体基因敲除小鼠中，肾上腺髓质素结合蛋白25（SNAP-25）的表达发生改变。

Adrenal gland SNAP-25 expression is altered in thyroid hormone receptor knock-out mice.

作者信息

Hepp R, Grant N J, Espliguero G, Aunis D, Sarlieve L L, Rodrigues-Pena A, Langley K

机构信息

INSERM Unité 338, Biologie de la Communication Cellulaire, Strasbourg, France.

出版信息

Neuroreport. 2001 May 25;12(7):1427-30. doi: 10.1097/00001756-200105250-00026.

DOI:10.1097/00001756-200105250-00026

PMID:11388423

Abstract

SNAP-25 is a protein in neurons and neuroendocrine cells, which is involved, together with syntaxin and VAMP, in neurotransmitter release and neurite outgrowth. Since the thyroid hormone receptors TR alpha and TR beta are essential for nervous system development, their possible role in regulating the expression of these vesicle trafficking proteins was examined by analysing SNAP-25 levels in TR alpha and TR beta knock-out mice. Immunoblotting and RT-PCR showed that SNAP-25 levels are increased in the adrenal gland, but not in cerebellum, in knock-out mice, while syntaxin-1 and VAMP-2 are unaffected in either tissue. Treatment of the pheochromocytoma-derived cell line PC12 with the thyroid hormone L-3,5,3'-triiodothyronine (T3) decreased SNAP-25 expression. Together, these data suggest that thyroid hormones exert a negative regulatory effect on SNAP-25 in adrenal medullary neuroendocrine cells.

摘要

SNAP-25是一种存在于神经元和神经内分泌细胞中的蛋白质，它与 syntaxin 和 VAMP 一起参与神经递质释放和神经突生长。由于甲状腺激素受体TRα和TRβ对神经系统发育至关重要，因此通过分析TRα和TRβ基因敲除小鼠中SNAP-25的水平，研究了它们在调节这些囊泡运输蛋白表达中的可能作用。免疫印迹和RT-PCR结果显示，基因敲除小鼠肾上腺中SNAP-25水平升高，但小脑未升高，而 syntaxin-1 和 VAMP-2 在两种组织中均未受影响。用甲状腺激素 L-3,5,3'-三碘甲状腺原氨酸（T3）处理嗜铬细胞瘤衍生细胞系PC12可降低SNAP-25表达。这些数据共同表明，甲状腺激素对肾上腺髓质神经内分泌细胞中的SNAP-25发挥负调节作用。