Rees M W, Short M N
Biochim Biophys Acta. 1975 May 30;393(1):15-23. doi: 10.1016/0005-2795(75)90211-1.
The amino acid sequence of the coat protein of the cowpea strain of tobacco mosaic virus (cowpea virus) has been determined. The tryptic peptide overlaps were obtained by digesting the protein with chymotrypsin and separating and analysing the lysine-and arginine-containing chymotryptic peptides. The primary structure of cowpea virus protein has been found to differ markedly from that of any other known strain of tobacco mosaic virus, and contains 3 amino acid residues more and 96 amino acid changes from the type strain. The significance of the distribution of those areas of the protein in which the amino acid residues are the same for all naturally occurring strains and chemically induced mutants of tobacco mosaic virus so far studied and the residues that form the important carboxyl-carboxylate pairs are discussed.
已确定烟草花叶病毒豇豆株系（豇豆病毒）外壳蛋白的氨基酸序列。通过用胰凝乳蛋白酶消化该蛋白，并分离和分析含赖氨酸和精氨酸的胰凝乳蛋白酶肽段，获得了胰蛋白酶肽段重叠区。已发现豇豆病毒蛋白的一级结构与任何其他已知的烟草花叶病毒株系明显不同，与典型株系相比多了3个氨基酸残基，且有96个氨基酸变化。文中讨论了对于迄今所研究的烟草花叶病毒所有天然株系和化学诱导突变体而言，该蛋白中氨基酸残基相同的区域以及形成重要羧基 - 羧酸盐对的残基的分布意义。
