Kahn L D, Witnauer L P
Biochim Biophys Acta. 1975 May 30;393(1):247-52. doi: 10.1016/0005-2795(75)90237-8.
Electric birefringence decay curves of collagen suspended in aqueous buffered media were plotted as functions of pulse width and amplitude. They were then resolved into two components by means of an analog simulator. When these data were combined with the results of repeated pulsing, it was shown that an electric field promotes aggregation of collagen, although the variety of aggregate sizes falls within a fixed range. Observations of electric birefringence of dissolved collagen preparations as a function of ionic strengths tend to indicate that the bonding that occurs in an electric field is electrostatic.
将悬浮于水性缓冲介质中的胶原蛋白的电双折射衰减曲线绘制为脉冲宽度和幅度的函数。然后通过模拟模拟器将它们分解为两个成分。当这些数据与重复脉冲的结果相结合时,结果表明电场促进了胶原蛋白的聚集,尽管聚集体大小的种类落在一个固定范围内。对溶解的胶原蛋白制剂的电双折射作为离子强度的函数的观察倾向于表明在电场中发生的键合是静电键合。