Lin S X, Grant B, Hirsh D, Maxfield F R
Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10021, USA.
Nat Cell Biol. 2001 Jun;3(6):567-72. doi: 10.1038/35078543.
RME-1 is an Eps15-homology (EH)-domain protein that was identified in a genetic screen for endocytosis genes in Caenorhabditis elegans. When expressed in a CHO cell line, the worm RME-1 protein and a mouse homologue are both associated with the endocytic recycling compartment. Here we show that expression of a dominant-negative construct with a point mutation near the EH domain results in redistribution of the endocytic recycling compartment and slowing down of transferrin receptor recycling. The delivery of a TGN38 chimaeric protein to the trans-Golgi network is also slowed down. The function of Rme-1 in endocytic recycling is evolutionarily conserved in metazoans as shown by the protein's properties in C. elegans.
RME-1是一种Eps15同源（EH）结构域蛋白，它是在秀丽隐杆线虫内吞作用基因的遗传筛选中被鉴定出来的。当在CHO细胞系中表达时，线虫的RME-1蛋白和一种小鼠同源物都与内吞循环区室相关。我们在此表明，在EH结构域附近具有点突变的显性负性构建体的表达会导致内吞循环区室的重新分布以及转铁蛋白受体循环的减慢。TGN38嵌合蛋白向反式高尔基体网络的转运也会减慢。如Rme-1蛋白在秀丽隐杆线虫中的特性所示，Rme-1在内吞循环中的功能在后生动物中是进化保守的。
