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真核生物核糖体蛋白。兔肝亚基蛋白质的分子量。

Eukaryotic ribosomal proteins. Molecular weights of proteins from rabbit liver subunits.

作者信息

Creusot F, Delaunay J, Schapira G

出版信息

Biochimie. 1975;57(2):167-73. doi: 10.1016/s0300-9084(75)80167-2.

DOI:10.1016/s0300-9084(75)80167-2
PMID:1138974
Abstract

The molecular weights of the proteins from rabbit liver ribosomal 40 S and 60 S subunits were determined after preliminary separation of these proteins by two-dimensional electrophoresis: each spot present in the polyacrylamide slab was cut off, eluted and rerun in a SDS one-dimensional polyacrylamide gel. The molecular weights range from 9,000 to 35,000 with a number-average molecular weight of 19,600 for the 40 S proteins, and from 9,400 to 52,000 with a number-average molecular weight of 23,600 for 60 S proteins.

摘要

通过二维电泳对兔肝核糖体40S和60S亚基的蛋白质进行初步分离后，测定了这些蛋白质的分子量：将聚丙烯酰胺板上的每个斑点切下、洗脱，并在SDS一维聚丙烯酰胺凝胶中重新运行。40S蛋白质的分子量范围为9000至35000，数均分子量为19600；60S蛋白质的分子量范围为9400至52000，数均分子量为23600。

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