大鼠肝脏双链RNA的细胞核及线粒体起源

Nuclear and mitochondrial origin of rat liver double-stranded RNA.

作者信息

Harel L, Riou G, Montagnier L

出版信息

Biochimie. 1975;57(2):227-33. doi: 10.1016/s0300-9084(75)80169-6.

Abstract

We have studied the intracellular location of double-stranded RNA from rat liver. The majority of this dsRNA is associated with nuclear and mitochondrial fractions and the remaining portion is associated with microsomes. Mitochondrial dsRNA hybridizes specifically with purified strands of mitochondrial DNA and seems therefore to arise from transcription of the latter. A fraction of nuclear dsRNA hybridizes with repetitive sequences of nuclear DNA. Because of self-hybridization of dsRNA strands, it was not possible to determine whether another fraction of DSRNA is homologous to nonrepetitive sequences of DNA. Both mitochondrial and nuclear types of dsRNA are not retained on oligo(dT) cellulose columns and therefore seem to lack long poly A sequences. The mechanism of formation of dsRNA is discussed.

摘要

我们研究了来自大鼠肝脏的双链RNA的细胞内定位。这种双链RNA的大部分与细胞核和线粒体部分相关联，其余部分与微粒体相关联。线粒体双链RNA与纯化的线粒体DNA链特异性杂交，因此似乎是由后者转录产生的。一部分细胞核双链RNA与细胞核DNA的重复序列杂交。由于双链RNA链的自我杂交，无法确定另一部分双链RNA是否与DNA的非重复序列同源。线粒体和细胞核类型的双链RNA都不能保留在寡聚（dT）纤维素柱上，因此似乎缺乏长聚腺苷酸序列。本文讨论了双链RNA的形成机制。