McPherson P S, Kay B K, Hussain N K
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, H3A 2B4, Canada.
Traffic. 2001 Jun;2(6):375-84. doi: 10.1034/j.1600-0854.2001.002006375.x.
Ligand binding to receptor tyrosine kinases and G-protein-coupled receptors initiates signal transduction events and induces receptor endocytosis via clathrin-coated pits and vesicles. While receptor-mediated endocytosis has been traditionally considered an effective mechanism to attenuate ligand-activated responses, more recent studies demonstrate that signaling continues on the endocytic pathway. In fact, certain signaling events, such as the activation of the extracellular signal-regulated kinases, appear to require endocytosis. Protein components of signal transduction cascades can assemble at clathrin coated pits and remain associated with endocytic vesicles following their dynamin-dependent release from the plasma membrane. Thus, endocytic vesicles can function as a signaling compartment distinct from the plasma membrane. These observations demonstrate that endocytosis plays an important role in the activation and propagation of signaling pathways.
配体与受体酪氨酸激酶和G蛋白偶联受体的结合引发信号转导事件，并通过网格蛋白包被的小窝和囊泡诱导受体内吞作用。虽然传统上认为受体介导的内吞作用是减弱配体激活反应的有效机制，但最近的研究表明，信号传导在内吞途径上仍会继续。事实上，某些信号事件，如细胞外信号调节激酶的激活，似乎需要内吞作用。信号转导级联反应的蛋白质成分可以在网格蛋白包被的小窝处组装，并在其从质膜上通过发动蛋白依赖性释放后仍与内吞囊泡相关联。因此，内吞囊泡可以作为一个与质膜不同的信号区室发挥作用。这些观察结果表明，内吞作用在信号通路的激活和传播中起着重要作用。
