Mazin A L, Sulimova G E
Biokhimiia. 1975 Jan-Feb;40(1):115-22.
A modification of the classical method of hydroxyapatite synthesis is proposed. The essence of the modification is hydroxyapatite synthesis in the presence of an additional component silicic acid particles. The subsequent steps of the method are modified so, as to retain the intactness of crystals at all the stages of preparation and use of the adsorbent. The final product consists of large spherical agregates (200-250 mu in diameter) and contains about 1% of tightly bound silicic acid. It slightly differs from usual hydroxyapatite in its chromatographic properties. Granulated hydroxyapatite obtained has a high specific capacity and can be repeatedly used in experiments (up to 50 chromatographic cycles). Native high-polymeric T2 phage DNA was practically quantitatively eluated from the column. Conditions for chromatography of some proteins (lysozyme, RNase, DNase) are described. Fractionation and purification of T2 and T3 bacteriophages and TMV are carried out by means of chromatography on granulated hydroxyapatite.
提出了一种对经典羟基磷灰石合成方法的改进。改进的实质是在额外的硅酸颗粒存在下合成羟基磷灰石。该方法的后续步骤进行了改进，以便在吸附剂制备和使用的所有阶段都能保持晶体的完整性。最终产物由大的球形聚集体（直径200 - 250微米）组成，含有约1%紧密结合的硅酸。其色谱性质与普通羟基磷灰石略有不同。所获得的粒状羟基磷灰石具有高比容量，可在实验中重复使用（多达50个色谱循环）。天然高分子量T2噬菌体DNA几乎能从柱上定量洗脱。描述了一些蛋白质（溶菌酶、核糖核酸酶、脱氧核糖核酸酶）的色谱条件。通过在粒状羟基磷灰石上进行色谱法对T2和T3噬菌体以及烟草花叶病毒进行分离和纯化。
