Phillips A R, Abu-Zidan F M, Farrant G J, Zwi J L, Cooper G J, Windsor J A
Pancreatitis Research Group, Department of Surgery, University of Auckland, Auckland, New Zealand.
Surgery. 2001 Jun;129(6):730-5. doi: 10.1067/msy.2001.113892.
Previous work has demonstrated that intestinal ischemia increases plasma amylin concentration. This study examined the relationship between the degree of intestinal ischemia injury and plasma amylin in an experimental rat model.
Wistar rats were divided into a control group (n = 6); a sham-operated group (n = 9); and 3 intestinal ischemia-reperfusion groups (n = 8 in each), which underwent clamping of the superior mesenteric artery for either 15, 30, or 45 minutes followed by 15 minutes of reperfusion. Samples were then collected for intestinal histology and measurement of amylin, insulin, and glucose.
There was a positive correlation between the histologic score of the intestinal injury and the measured plasma amylin concentration (R = 0.48, P =.007). The median plasma concentration of amylin was 62 pmol/L (range, 42-97 pmol/L) in the 30-minute intestinal ischemia group and 58 pmol/L (42-86 pmol/L) in the 45-minute intestinal ischemia group. Both these groups were increased compared with the sham-operated group (29 pmol/L; range, 22-57 pmol/L; P <.001 and P <.005, respectively) and the control group (28 pmol/L; range, 26-42 pmol/L; P <.001 and P <.0005, respectively). The median plasma concentration of insulin in the 30-minute intestinal ischemia group was 4230 pmol/L (range, 1360-5770 pmol/L), which was increased compared with both the control group (950 pmol/L; range, 550-1510 pmol/L; P <.005) and the sham-operated group (720 pmol/L; range, 280-4180 pmol/L; P<.005). There were no differences between any of the other groups either for glucose, insulin, or amylin.
Plasma amylin concentration is related to the severity of intestinal ischemic injury.
先前的研究表明，肠道缺血会增加血浆胰淀素浓度。本研究在实验大鼠模型中探讨了肠道缺血损伤程度与血浆胰淀素之间的关系。
将Wistar大鼠分为对照组（n = 6）；假手术组（n = 9）；以及3个肠道缺血再灌注组（每组n = 8），对肠系膜上动脉进行15、30或45分钟的夹闭，随后再灌注15分钟。然后收集样本进行肠道组织学检查以及胰淀素、胰岛素和葡萄糖的测量。
肠道损伤的组织学评分与测得的血浆胰淀素浓度之间存在正相关（R = 0.48，P = 0.007）。30分钟肠道缺血组血浆胰淀素的中位数浓度为62 pmol/L（范围为42 - 97 pmol/L），45分钟肠道缺血组为58 pmol/L（42 - 86 pmol/L）。与假手术组（29 pmol/L；范围为22 - 57 pmol/L；P < 0.001和P < 0.005）及对照组（28 pmol/L；范围为26 - 42 pmol/L；P < 0.001和P < 0.0005）相比，这两组均升高。30分钟肠道缺血组血浆胰岛素的中位数浓度为4230 pmol/L（范围为1360 - 5770 pmol/L），与对照组（950 pmol/L；范围为550 - 1510 pmol/L；P < 0.005）和假手术组（720 pmol/L；范围为280 - 4180 pmol/L；P < 0.005）相比均升高。在葡萄糖、胰岛素或胰淀素方面，其他任何组之间均无差异。
血浆胰淀素浓度与肠道缺血损伤的严重程度相关。
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