Robinson B H, Oei J
Can J Biochem. 1975 May;53(5):643-7. doi: 10.1139/o75-089.
Guinea pig heart mitochondria loaded with [-14C]citrate show exchanges of radioactivity at 30 degrees C with added citrate, L-malate and phosphoenolpyruvate. These exchanges are inhibited by benzene-1,2,3-tricarboxylate. Measurements of rates of citrate transport indicate that the activity of this transporting system is low in heart mitochondria compared to that observed in liver mitochondria. The K(m) values obtained indicate a similarity to those obtained in liver. Citrate oxidation by coupled mitochondria was also found to be slow at 30 degrees C but was inhibited by benzene-1,2,3-tricarboxylate. The role of mitochondrial citrate transport in control of glycolytic flux in the heart is discussed.
装载有[-14C]柠檬酸盐的豚鼠心脏线粒体在30℃下会与添加的柠檬酸盐、L-苹果酸和磷酸烯醇丙酮酸发生放射性交换。这些交换受到苯-1,2,3-三羧酸盐的抑制。柠檬酸盐转运速率的测量表明，与肝脏线粒体相比，该转运系统在心脏线粒体中的活性较低。所获得的米氏常数（K(m)）值表明与在肝脏中获得的值相似。还发现，在30℃下，偶联线粒体对柠檬酸盐的氧化作用缓慢，但受到苯-1,2,3-三羧酸盐的抑制。本文讨论了线粒体柠檬酸盐转运在心脏糖酵解通量控制中的作用。
