Bociek S M, Welti D
Carbohydr Res. 1975 Jul;42(2):217-26. doi: 10.1016/s0008-6215(00)84263-9.
I.r. absorption bands associated with the functional groups of carboxylic acid derivatives are useful for the analysis of alginates and pectins. The ester, amide, and uronate contents of pectins and the uronate content of alginates were determined, respectively, from the ester-carbonyl stretching band (1740 cm- minus 1), the amide I band (1650 cm- minus 1), and the carboxylate antisymmetric stretching band (1607 cm- minus 1) obtained from the spectra of solutions in D2O-phosphate buffer. The results are accurate to within plus or minus 2-4%, are self consistent, and agree well with the few reliable results that are available. The method should be applicable for the determination of carboxylic acid derivatives in other polysaccharides.
与羧酸衍生物官能团相关的红外吸收带可用于藻酸盐和果胶的分析。分别从D2O - 磷酸盐缓冲溶液光谱中获得的酯羰基伸缩带（1740 cm⁻¹）、酰胺I带（1650 cm⁻¹）和羧酸盐反对称伸缩带（1607 cm⁻¹）来测定果胶的酯、酰胺和糖醛酸含量以及藻酸盐的糖醛酸含量。结果的准确度在±2 - 4%以内，具有自洽性，并且与现有的少数可靠结果吻合良好。该方法应适用于其他多糖中羧酸衍生物的测定。
