Inase N, Miyake S, Yoshizawa Y
Department of Pulmonary Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 1 13-8519, Japan.
Anticancer Res. 2001 Mar-Apr;21(2A):1111-4.
To achieve specific and efficient expression of transfected suicide gene in malignant mesothelioma cells, we explored the 5-flanking region (2.2 kb) of the calretinin gene as a tumor-specific promoter. RT-PCR analysis demonstrated the expression of calretinin mRNA in two human malignant mesothelioma cell lines (H2052 and 211 H). A reporting vector containing the calretinin promoter (pCAL-LUC) exhibited relatively high luciferase activity in these cell lines. After transfecting an expression vector containing the calretinin promoter-bound thymidine kinase gene (pCAL-TK) into the cells, we examined their sensitivity to ganciclovir (GCV). In the mesothelioma cell lines, the pCAL-TK transfected cells became about 100 times more sensitive to GCV than the parental cells. The calretinin promoter seems to be a promising candidate as a specific and efficient promoter in malignant mesothelioma.
为了在恶性间皮瘤细胞中实现转染自杀基因的特异性高效表达，我们探索了钙视网膜蛋白基因的5'侧翼区（2.2 kb）作为肿瘤特异性启动子。逆转录聚合酶链反应（RT-PCR）分析表明，钙视网膜蛋白mRNA在两个人类恶性间皮瘤细胞系（H2052和211H）中表达。含有钙视网膜蛋白启动子的报告载体（pCAL-LUC）在这些细胞系中表现出相对较高的荧光素酶活性。将含有与钙视网膜蛋白启动子结合的胸苷激酶基因的表达载体（pCAL-TK）转染到细胞中后，我们检测了它们对更昔洛韦（GCV）的敏感性。在间皮瘤细胞系中，转染了pCAL-TK的细胞对GCV的敏感性比亲代细胞高约100倍。钙视网膜蛋白启动子似乎是恶性间皮瘤中一种有前景的特异性高效启动子候选者。
