顺铂（CDDP）应用后豚鼠前庭中单链DNA（ssDNA）的检测。
Detection of single-stranded DNA (ssDNA) in the vestibule of guinea pigs after the application of cisplatinum (CDDP).
作者信息
Watanabe K, Jinnouchi K, Yagi T
机构信息
Department of Oto-Rhino-Laryngology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8603, Japan.
出版信息
Anticancer Res. 2001 Mar-Apr;21(2A):1135-8.
BACKGROUND
Cisplatinum (CDDP) has a toxic effect on the inner ear. CDDP reacts with DNA and leads to apoptotic cell death. In this study, the presence of single-stranded DNA (ssDNA) was examined immunohistochemically.
MATERIALS AND METHODS
CDDP (10 mg/kg b.w.) was injected into the guinea pigs intra-peritoneally. Animals were sacrificed three days after the injection. The temporal bones were fixed via cardiac infusion. The tissues were embedded in paraffin and then used for immunohistochemical examination.
RESULTS
That ssDNA was detected in the CDDP treated vestibule. The sensory epithelium, transitional area, dark cells and vestibular ganglion cells exhibited a positive immunoreactivity to ssDNA.
CONCLUSIONS
DNA fragmentation is a characteristic feature of apoptosis. Fragmented DNA is detected as ssDNA. Our results indicate that CDDP involves the apoptosis in the inner ear and this is the cause of the ototoxicity of CDDP.
背景
顺铂（CDDP）对内耳具有毒性作用。CDDP与DNA发生反应并导致细胞凋亡性死亡。在本研究中，采用免疫组织化学方法检测单链DNA（ssDNA）的存在情况。
材料与方法
将CDDP（10mg/kg体重）腹腔注射到豚鼠体内。注射三天后处死动物。通过心脏灌注固定颞骨。将组织包埋于石蜡中，然后用于免疫组织化学检查。
结果
在经CDDP处理的前庭中检测到ssDNA。感觉上皮、过渡区、暗细胞和前庭神经节细胞对ssDNA呈现阳性免疫反应。
结论
DNA片段化是细胞凋亡的一个特征性表现。片段化的DNA以ssDNA形式被检测到。我们的结果表明，CDDP参与内耳的细胞凋亡，这是CDDP耳毒性的原因。
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