Zebrowska G, Lewa C J, Ramee M P, Husson F, De Certaines J D
Laboratoire de Résonance Magnétique en Biologie et Médecine, Université de Rennes I, 35043 Rennes, France.
Anticancer Res. 2001 Mar-Apr;21(2A):1213-7.
It was our aim to evaluate the potential of proton relaxation times for the early detection of radiation-induced spleen changes.
Female Swiss mice were irradiated with doses ranging from 0.05 Gy to 4 Gy. The body weight, the spleen weight and the spleen water content of single animals were determined. Measurements of longitudinal (T1) and transversal (T2) proton relaxation times of the spleen samples were performed in a 0.47 T spectrometer. Histological examinations of the control and irradiated organs were performed.
NMR measurements during the first five days after irradiation showed that total body gamma-irradiation with doses from 1.5 Gy to 4 Gy results in decreasing T1 of the murine spleen. Significant shortening in T2 was observed for the spleen of animals irradiated with a dose of 4 Gy. Histological examinations demonstrated subnormal architecture in slices derived from animals irradiated with 2 Gy and 4 Gy.
The fluctuations of the spleen T1 and T2 of irradiated mice are correlated with relative spleen weight and can be used to estimate radiation induced changes in this organ.
我们的目的是评估质子弛豫时间在早期检测辐射诱导的脾脏变化方面的潜力。
用0.05 Gy至4 Gy的剂量对雌性瑞士小鼠进行辐照。测定单只动物的体重、脾脏重量和脾脏含水量。在0.47 T的光谱仪中对脾脏样本的纵向（T1）和横向（T2）质子弛豫时间进行测量。对对照和辐照后的器官进行组织学检查。
辐照后前五天的核磁共振测量显示，全身γ辐照剂量为1.5 Gy至4 Gy会导致小鼠脾脏的T1降低。接受4 Gy剂量辐照的动物脾脏的T2出现显著缩短。组织学检查表明，来自接受2 Gy和4 Gy辐照动物的切片结构异常。
辐照小鼠脾脏T1和T2的波动与脾脏相对重量相关，可用于估计该器官的辐射诱导变化。
