对受刺激增殖的WI-38成纤维细胞染色质的圆二色性和溴化乙锭结合研究。

Circular dichroism and ethidium bromide binding studies of chromatin from WI-38 fibroblasts stimulated to proliferate.

作者信息

Nicolini C, Baserga R

出版信息

Chem Biol Interact. 1975 Aug;11(2):101-16. doi: 10.1016/0009-2797(75)90017-4.

Abstract

Confluent monolayers of WI-38 diploid fibroblasts can be stimulated to proliferate by fresh serum. In the first 3 h after stimulation (that is, several hours before DNA replication) the chromatin of stimulated cells show structrual changes which include: (1) an increase in maximum positive ellipticity and a blue shift in the 250-300 nm region of circular dichroism spectra; and (2) an increase,in isolated chromatin, of the number of binding sites for the intercalating dye, ethidium bromide.The differences between chromtin of stimulated and chromatin of unstimulated cells are abolised when bother chromatins are treated with 0.25 M NaCL.

摘要

WI-38 二倍体成纤维细胞的融合单层可被新鲜血清刺激增殖。在刺激后的最初 3 小时内（即 DNA 复制前的几个小时），受刺激细胞的染色质会出现结构变化，包括：（1）最大正椭圆率增加，以及圆二色光谱在 250 - 300 nm 区域出现蓝移；（2）在分离的染色质中，嵌入染料溴化乙锭的结合位点数量增加。当用 0.25 M NaCl 处理受刺激细胞和未受刺激细胞的染色质时，两者染色质之间的差异消失。