Lewis C E, Doyle J J
L. H. Bailey Hortorium, Cornell University, Ithaca, New York 14853, USA.
Mol Phylogenet Evol. 2001 Jun;19(3):409-20. doi: 10.1006/mpev.2001.0932.
There is currently a shortage of DNA regions known to be useful for phylogenetic research in palms (Arecaceae). We report the development and use of primers for amplifying and sequencing regions of the nuclear gene malate synthase. In palms the gene appears to be single-copy, with exon regions that are phylogenetically informative within the family. We constructed a phylogeny of 45 palms and five outgroup taxa using 428 bp of malate synthase exon regions. We found that some major clades within the family were recovered, but there was a lack of resolution among the genera in subfamilies Arecoideae, Ceroxyloideae, Coryphoideae, and Phytelephantoideae. In a second analysis, malate synthase exon regions totaling 1002 bp were sequenced for 16 palms and two outgroup taxa. There was increased bootstrap support for some groups and for the placement of the monotypic genus Nypa as sister to the rest of the family. A comparison with data sets from noncoding regions of the chloroplast genome indicates that malate synthase sequences are more variable and potentially contain more phylogenetic information. We found no evidence of multiple copies of the malate synthase gene in palm genomes.
目前已知可用于棕榈科（槟榔科）系统发育研究的DNA区域短缺。我们报告了用于扩增和测序核基因苹果酸合酶区域的引物的开发和应用。在棕榈科中，该基因似乎是单拷贝的，其外显子区域在科内具有系统发育信息。我们利用428个碱基对的苹果酸合酶外显子区域构建了45种棕榈和5个外类群分类单元的系统发育树。我们发现该科内的一些主要分支被识别出来，但在槟榔亚科、蜡椰亚科、贝叶棕亚科和鱼尾葵亚科的属之间缺乏分辨率。在第二项分析中，对16种棕榈和2个外类群分类单元的总共1002个碱基对的苹果酸合酶外显子区域进行了测序。一些类群以及单型属水椰作为该科其他类群的姐妹群的位置得到了更高的自展支持。与叶绿体基因组非编码区域的数据集比较表明，苹果酸合酶序列更具变异性，可能包含更多的系统发育信息。我们在棕榈基因组中未发现苹果酸合酶基因多拷贝的证据。
