Lacolley P, Challande P, Boumaza S, Cohuet G, Laurent S, Boutouyrie P, Grimaud J A, Paulin D, Lamazière J M, Li Z
Institut National de la Santé et de la Recherche Médicale, INSERM U337, 15 Rue de l'Ecole de Médecine, 75270 Paris Cedex 06, France.
Cardiovasc Res. 2001 Jul;51(1):178-87. doi: 10.1016/s0008-6363(01)00278-4.
Our aim was to determine in desmin homozygous mutant mice the viscoelastic properties, the mechanical strength and the structure of the carotid artery.
To assess the viscoelastic properties of large arteries, we have performed an in vivo analysis of the diameter-, and distensibility-pressure curves of the common carotid artery (CCA) in homozygous (Des -/-), heterozygous (Des +/-) and wild-type (Des +/+) mice. To evaluate the mechanical strength, we have measured the in vitro intraluminal pressure producing the rupture of the carotid artery wall. The structure analysis of the arterial wall was based on histology and electronic microscopy.
A lower distensibility and an increase of arterial wall viscosity were observed in Des -/- compared with Des +/+. Arterial thickness of Des -/- was similar to those of Des +/+, without changes in elastin and collagen contents. Electron microscopy revealed that the perimeter of cellular fingerlike-projections was smaller in Des -/-, indicating that the cells have lost part of their connections to the extracellular matrix. The rupture pressure was significantly lower in Des -/- (1500+/-200 mmHg) compared with Des +/+ (2100+/-80 mmHg) indicating a lower mechanical strength of the vascular wall. No significant difference was found between Des +/- and Des +/+.
The desmin is essential to maintain proper viscoelastic properties, structure and mechanical strength of the vascular wall.
我们的目的是确定结蛋白纯合突变小鼠颈动脉的粘弹性特性、机械强度和结构。
为了评估大动脉的粘弹性特性，我们对纯合子（Des -/-）、杂合子（Des +/-）和野生型（Des +/+）小鼠的颈总动脉（CCA）的直径-和扩张性-压力曲线进行了体内分析。为了评估机械强度，我们测量了导致颈动脉壁破裂的体外腔内压力。动脉壁的结构分析基于组织学和电子显微镜检查。
与Des +/+相比，Des -/-的扩张性较低，动脉壁粘度增加。Des -/-的动脉厚度与Des +/+相似，弹性蛋白和胶原蛋白含量无变化。电子显微镜显示，Des -/-中细胞指状突起的周长较小，表明细胞与细胞外基质的部分连接丧失。与Des +/+（2100±80 mmHg）相比，Des -/-的破裂压力显著较低（1500±200 mmHg），表明血管壁的机械强度较低。Des +/-和Des +/+之间未发现显著差异。
结蛋白对于维持血管壁适当的粘弹性特性、结构和机械强度至关重要。
