Bomfim-Patrício M C., Salatino A, Martins A B., Wurdack J J., Salatino M L.F.
Institute of Biosciences, University of São Paulo,C. Postal 11461, CEP 05422-970, SP, São Paulo, Brazil
Biochem Syst Ecol. 2001 Jul;29(7):711-726. doi: 10.1016/s0305-1978(00)00116-2.
Leaves of species of three closely related genera of Melastomataceae, Lavoisiera, Microlicia and Trembleya were analyzed for their flavonoid composition. A total of 116 compounds were obtained, comprising 69 flavonol and 47 flavone glycosides. The common occurrence of flavones, including 6-oxygenated derivatives, characterize Lavoisiera (which often yielded methoxylated flavonols and flavones). Flavonols predominate in species of Microlicia. The flavonoid patterns of Trembleya are rather confusing, some species being akin to Lavoisiera, others to Microlicia. UPGMA analysis using the flavonoid aglycones as characters and the samples analyzed as OTUs gives no complete resolution for the three genera, but provides clusters combining exclusively or preferentially species of either Lavoisiera and Microlicia. Species of Trembleya emerge from the phenogram interspersed among species of the other genera. The data suggest the recognition of Lavoisiera and Microlicia and the lumping of species of Trembleya with either of these genera. If greater flavone diversity is viewed as indicative of further evolutionary advancement, shrubby habits in Microliceae (Lavoisiera and Trembleya) should be regarded as derived from herbaceous ones (Microlicia).
对野牡丹科三个近缘属——Lavoisiera属、Microlicia属和Trembleya属的物种叶片进行了黄酮类成分分析。共获得116种化合物，包括69种黄酮醇和47种黄酮苷。黄酮类化合物（包括6-氧化衍生物）的普遍存在是Lavoisiera属的特征（该属常产生甲氧基化黄酮醇和黄酮）。黄酮醇在Microlicia属的物种中占主导地位。Trembleya属的黄酮类模式相当混乱，一些物种类似于Lavoisiera属，另一些则类似于Microlicia属。以黄酮苷元为特征、以所分析的样本为操作分类单元进行的UPGMA分析，无法完全区分这三个属，但提供了仅由或优先由Lavoisiera属和Microlicia属的物种组成的聚类。Trembleya属的物种出现在系统发育图中，散布于其他属的物种之间。数据表明应认可Lavoisiera属和Microlicia属，并将Trembleya属的物种与这两个属中的任一个合并。如果认为更高的黄酮多样性表明进一步的进化进步，那么微药野牡丹族（Lavoisiera属和Trembleya属）中的灌木习性应被视为源自草本习性（Microlicia属）。
