去甲肾上腺素通过α-肾上腺素能受体机制刺激淋巴细胞ATP酶。

Norepinephrine stimulation of lymphocyte ATPase by an alpha adrenergic receptor mechanism.

作者信息

Coffey R G, Hadden E M, Hadden J W

出版信息

Endocr Res Commun. 1975;2(2):179-98. doi: 10.1080/07435807509053848.

Abstract

The effects of norepinephrine in interaction with adrenergic blocking compounds were studied on membrane adenosine triphosphatase (ATPase) activities of human lymphocytes and lymphoblasts. Sodium-potassium ion exchange pump activity was assayed by 86-Rb uptake and ATPase activity of membrane fractions was assayed by ADP and inorganic phosphate generation. The results of these studies indicate that norepinephrine acts by an alpha adrenergic mechanism to enhance membrane sodium-potassium ion exchange pump activity and ATPase activity. The pharmacologic and ionic dissection of the adrenergic sensitivity of ATPase activity indicates that this alpha adrenergic mechanism is related to membrane ATPase activities in addition to that associated with the ion exchange pump. Analysis of fractions obtained by sucrose gradients indicates that the action of norepinephrine is localized in the plasma membrane. Beta adrenergic stimulation was observed to inhibit ATPase activity. The complexity of adrenergic effects on membrane ATPase suggests interactions of hormone modulation of membrane nucleotide cyclases and transport-related ATPase enzymes.

摘要

研究了去甲肾上腺素与肾上腺素能阻断化合物相互作用对人淋巴细胞和成淋巴细胞膜三磷酸腺苷酶（ATP酶）活性的影响。通过86-Rb摄取测定钠钾离子交换泵活性，通过ADP和无机磷酸盐生成测定膜组分的ATP酶活性。这些研究结果表明，去甲肾上腺素通过α肾上腺素能机制发挥作用，增强膜钠钾离子交换泵活性和ATP酶活性。对ATP酶活性的肾上腺素能敏感性进行药理和离子分析表明，这种α肾上腺素能机制除了与离子交换泵相关外，还与膜ATP酶活性有关。对蔗糖梯度分离得到的组分的分析表明，去甲肾上腺素的作用定位于质膜。观察到β肾上腺素能刺激抑制ATP酶活性。肾上腺素能对膜ATP酶作用的复杂性提示了膜核苷酸环化酶的激素调节与转运相关ATP酶之间的相互作用。