C组轮状病毒NSP4可诱导新生小鼠腹泻。

Group C rotavirus NSP4 induces diarrhea in neonatal mice.

作者信息

Sasaki S, Horie Y, Nakagomi T, Oseto M, Nakagomi O

机构信息

Department of Microbiology, Akita University School of Medicine, Akita, Japan.

出版信息

Arch Virol. 2001;146(4):801-6. doi: 10.1007/s007050170148.

DOI:10.1007/s007050170148

PMID:11402865

Abstract

Nonstructural glycoprotein NSP4 of group A rotavirus induces diarrhea in neonatal mice by functioning as an enterotoxin. Previously, our laboratory reported that the structural features of group A and group C rotavirus NSP4 proteins are well conserved despite a lack of sequence homology between group A and group C rotavirus NSP4 proteins [Horie Y, et al., Arch Virol (1997) 142: 1865-1872]. To test whether group C rotavirus NSP4 has an enterotoxigenic activity, we expressed in Escherichia coli the carboxy two-thirds (corresponding to amino acid residues 55-150) of the NSP4 protein derived from group C rotavirus strain Ehime 9301. This truncated NSP4 protein was able to induce diarrhea in 5-day-old CD-1 mice when administered intraperitoneally. Thus, group C rotavirus NSP4 acts as an enterotoxin like group A rotavirus NSP4.

摘要

A组轮状病毒的非结构糖蛋白NSP4作为一种肠毒素可诱导新生小鼠腹泻。此前，我们实验室报道，尽管A组和C组轮状病毒NSP4蛋白之间缺乏序列同源性，但它们的结构特征却高度保守[堀江洋等，《病毒学文献》（1997年）142: 1865 - 1872]。为了检测C组轮状病毒NSP4是否具有产肠毒素活性，我们在大肠杆菌中表达了源自C组轮状病毒爱媛9301株的NSP4蛋白的羧基端三分之二（对应于氨基酸残基55 - 150）。当腹腔注射时，这种截短的NSP4蛋白能够在5日龄的CD - 1小鼠中诱导腹泻。因此，C组轮状病毒NSP4与A组轮状病毒NSP4一样，可作为一种肠毒素发挥作用。